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KMID : 1035320150420030197
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Korean Society for Dental Materials
2015 Volume.42 No. 3 p.197 ~ p.206
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Epigallocatechin gallate coated on Ti surface stimulates differentiation of human dental pulp cells
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Kim Young-Seok
Park Hee-Chul
Lee Dong-Hee
Yang Hyeong-Cheol
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Abstract
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Epigallocatechin gallate (EGCG), a flavonoid which is found in green tea and red wine, is known to have an osteogenic activity. In this study, the effect of EGCG on the differentiation of human dental pulp cells (HDPCs) was investigated. The growth of HDPCs was suppressed when the cells were exposed to 60 μM EGCG during 5 days. EGCG enhanced ALP activity of HDPCs at concentrations ranging from 1 to 30 μM. Furthermore, the mRNA expression of osteocalcin (OCN) and dentin sialophosphoprotein (DSPP) in HDPCs was enhanced by exposure to EGCG. Extracellular matrix mineralization was also promoted by EGCG even in the absence of other odontogenic factors such as ascorbic acid and dexamethasone. Therefore, it is certain that EGCG is capable of inducing odontogenicity of dental pulp cells. In the experiment of biomimetic apatite coating of titanium discs, EGCG was successfully incorporated into apatite coats and released from the surfaces into culture media.
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KEYWORD
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Epigallocatechin gallate, Human dental pulp cell, Differentiation, Dentin, Pulp
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